Optimized for parallel extraction of DNA and RNA from ticks.
Product Details
- Application-specific Lysis Tubes with beads guarantee effective homogenization of the starting material
- Patented extraction chemistry with a stringent Lysis Buffer system and a novel Binding Buffer
- When combined with rapidSTRIPE assays, the kit serves as a highly sensitive tool for detecting tick-borne pathogens (including TBE and/or FSME)
The blackPREP Tick DNA/RNA Kit allows researchers to simultaneously isolate DNA and RNA from ticks. This is of particular interest when testing ticks for RNA viruses (such as TBE or FSME) in addition to bacterial pathogens. The kit contains application-specific Lysis Tubes, including beads that have been optimized (in terms of their characteristics and quantity) specifically for homogenizing ticks. Following a subsequent lysis process, the DNA is bound to one filter membrane and the RNA is bound to another. The nucleic acids are then washed and eluted into separate reaction vessels. Analytik Jena’s rapidSTRIPE assays can then be used in later applications as fast, uncomplicated tools for detecting nucleic acids from tick-borne pathogens. These ready-to-use assays can be used as highly specific tools for detecting FSME, Borrelia, Rickettsia, Anaplasma and Babesia, among other pathogens.
Specifications
Starting material
Ticks
Average purity
1,7 – 2,0
Extraction time
Less than 1 hour (including lysis)