- Modified guanidine isothiocyanate/phenol method for extracting RNA
- High-quality RNA with no degradation after just approx. 1 hour of prep time
- Suitable for a variety of different starting materials
Product Details
The innuSOLV RNA Reagent is a solution for efficiently isolating total RNA from various quantities of different starting materials (such as tissue samples, cells, bacterial cells, plants, etc.). The extraction method is based on a single-step, liquid-phase separation that saves a significant amount of time. The innuSOLV RNA Reagent contains a mixture of phenol and guanidine isothiocyanate in a monophasic solution. After adding chloroform and centrifuging, the homogenization product separates into three phases:
- A reddish, organic phase on the bottom
- A whitish intermediate phase
- A colorless liquid phase on top containing the RNA
The RNA is then precipitated by adding an alcohol. Extraction withthe innuSOLV RNA Reagent produces high-quality nucleic acids withno degradation that are available for a variety of different applicationsafter just 1 hour of prep time.
Specifications
Preperation time
Approx. 60 minutes
Starting material
- Tissue samples (100 mg)
- Monolayer cells
- Cell suspensions (of animal or plant origin; yeast or bacterial cells; max. 5 × 106)